Single-cell electro-mechanical shear flow deformability cytometry.

ABSTRACT: The complex structural and molecular features of a cell lead to a set of specific dielectric and mechanical properties which can serve as intrinsic phenotypic markers that enable different cell populations to be characterised and distinguished. We have developed a microfluidic technique that exploits non-contact shear flow deformability cytometry to simultaneously characterise both the electrical and mechanical properties of single cells at high speed. Cells flow along a microchannel and are deformed (elongated) to different degrees by the shear force created by a viscoelastic fluid and channel wall. The electrical impedance of each cell is measured using sets of integrated microelectrodes along two orthogonal axes to determine the shape change and thus the electrical deformability, togeth

SUBMITTER: Chen J 

PROVIDER: S-EPMC11582679 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature