Incorporation of regulatory DNA elements within a viral vector improves recombinant protein expression in plants.

ABSTRACT: Plants have significant potential as recombinant protein expression chassis, as they can produce complex post-translationally modified proteins that are unobtainable using prokaryotic production systems, with almost limitless scalability and substantially reduced costs relative to eukaryotic cell cultures. Transient protein expression reduces the time taken between transformation and recombinant protein extraction and purification, however low protein yields relative to conventional stable expression systems remain a major obstacle. Here, we have assessed the effectiveness of combining several established genetic components, including a promoter, 5' UTR, 3' UTR, double terminator, and matrix attachment region, to modify the TMV-based pJL-TRBO expression vector for improved recombinant prot

SUBMITTER: Coates RJ 

PROVIDER: S-EPMC11582814 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature