Next-Generation AUC: Analysis of Multiwavelength Analytical Ultracentrifugation Data.

ABSTRACT: We describe important advances in methodologies for the analysis of multiwavelength data. In contrast to the Beckman-Coulter XL-A/I ultraviolet-visible light detector, multiwavelength detection is able to simultaneously collect sedimentation data for a large wavelength range in a single experiment. The additional dimension increases the data density by orders of magnitude, posing new challenges for data analysis and management. The additional data not only improve the statistics of the measurement but also provide new information for spectral characterization, which complements the hydrodynamic information. New data analysis and management approaches were integrated into the UltraScan software to address these challenges. In this chapter, we describe the enhancements and benefits realized

SUBMITTER: Gorbet GE 

PROVIDER: S-EPMC11583009 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature