Cellulose degradation in Glenea cantor (Fabricius) (Coleoptera: Cerambycidae): functional characterization of GcEGaseZ7 and Cellulase reveals a novel enzymatic activity.

ABSTRACT: Glenea cantor (Fabricius) is an important forest pest that mainly attacks kapok trees, breaking down cellulose and lignin through 3 enzyme activities: endoglucanase, filter paper enzyme, and cellobiase. In this study, we unveiled the cloning and expression of 10 endoglucanase genes, GcEGase5A1, GcEGase5A2, GcEGaseZ2, GcEGaseZ3, GcEGaseZ4, GcEGaseZ5, GcEGaseZ7, GcEGaseZ8, GcEGaseZ9, and Cellulase, all of which exhibit enzymatic activities in G. cantor. These findings indicated that Cellulase shares sequence homology with beetle GHF45, whereas the other 9 endoglucanase genes are homologous to beetle GHF5. GcEGaseZ4 presented the highest expression in the foregut. In contrast, GcEGase5A2 and Cellulase presented peak expression in the midgut. Furthermore, GcEGaseZ7 was identified as the most h

SUBMITTER: Su RR 

PROVIDER: S-EPMC11583219 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature