Quantifying defective and wild-type viruses from high-throughput RNA sequencing.

ABSTRACT:

Motivation

Defective viral genomes (DVGs) are variants of the wild-type (wt) virus that lack the ability to complete autonomously an infectious cycle. However, in the presence of their parental (helper) wt virus, DVGs can interfere with the replication, encapsidation, and spread of functional genomes, acting as a significant selective force in viral evolution. DVGs also affect the host's immune responses and are linked to chronic infections and milder symptoms. Thus, identifying and characterizing DVGs is crucial for understanding infection prognosis. Quantifying DVGs is challenging due to their inability to sustain themselves, which makes it difficult to distinguish them from the helper virus, especially using high-throughput RNA sequencing. An accurate quantification is essential

SUBMITTER: Munoz-Sanchez JC 

PROVIDER: S-EPMC11583936 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature