Advanced soft tissue visualization in conjunction with bone structures using contrast-enhanced micro-CT.

ABSTRACT:

Purpose

Micro-computed tomography (CT) analysis of soft tissues alongside bone remains challenging due to significant differences in X-ray absorption, preventing spatial inspection of bone remodeling including the cellular intricacies of mineralized tissues in developmental biology and pathology. The goal was to develop a protocol for contrast-enhanced micro-CT imaging that effectively visualizes soft tissues and cells in conjunction with bone while minimizing bone attenuation by decalcification.

Approach

Murine femur samples were decalcified in ethylenediaminetetraacetic acid and treated with three different contrast agents: (i) iodine in ethanol, (ii) phosphotungstic acid in water, and (iii) Lugol's iodine. Micro-CT scans were performed in the laboratory setup SkyScan 1172

SUBMITTER: Hildebrand T 

PROVIDER: S-EPMC11584031 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature