Whole blood coagulation in an ex vivo thrombus is sufficient to induce clot neutrophils to adopt a myeloid-derived suppressor cell signature and shed soluble Lox-1.

ABSTRACT:

Background

Blood clots are living tissues that release inflammatory mediators including IL-8/CXCL8 and MCP-1/CCL2. A deeper understanding of blood clots is needed to develop new therapies for prothrombotic disease states and regenerative medicine.

Objectives

To identify a common transcriptional shift in cultured blood clot leukocytes.

Methods

Differential gene expression of whole blood and cultured clots (4 hours at 37 °C) was assessed by RNA sequencing (RNAseq), reverse transcriptase-polymerase chain reaction, proteomics, and histology (23 diverse healthy human donors). Cultured clot serum bioactivity was tested in endothelial barrier functional assays.

Results

All cultured clots developed a polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC) signatur

SUBMITTER: Leonard J 

PROVIDER: S-EPMC11584067 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature