Colorimetric enzymatic rapid test for the determination of atropine in baby food using a smartphone.

ABSTRACT: A method for the enzymatic determination of atropine has been developed, which is based on a sequence of reactions involving (1) the hydrolysis of atropine to give tropine; (2) the enzymatic oxidation of tropine with NAD (catalysed by tropinone reductase); and (3) an indicator reaction, in which the NADH previously formed reduces the dye iodonitrotetrazolium chloride (INT) to a reddish species, the reaction catalysed by diaphorase. The method was first developed in solution (linear response range from 2.4 × 10^-6 M to 1.0 × 10^-4 M). It was then implemented in cellulose platforms to develop a rapid test where the determination is made by measuring the RGB coordinates of the platforms using a smartphone-based device. The device is based on the integrating sphere concept

SUBMITTER: Dominguez M 

PROVIDER: S-EPMC11584467 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature