Viral sequence determines HLA-E-restricted T cell recognition of hepatitis B surface antigen.

ABSTRACT: The non-polymorphic HLA-E molecule offers opportunities for new universal immunotherapeutic approaches to chronic infectious diseases. Chronic Hepatitis B virus (HBV) infection is driven in part by T cell dysfunction due to elevated levels of the HBV envelope (Env) protein hepatitis B surface antigen (HBsAg). Here we report the characterization of three genotypic variants of an HLA-E-binding HBsAg peptide, Env_371-379, identified through bioinformatic predictions and verified by biochemical and cellular assays. Using a soluble affinity-enhanced T cell receptor (TCR) (a09b08)-anti-CD3 bispecific molecule to probe HLA-E presentation of the Env_371-379 peptides, we demonstrate that only the most stable Env_371-379 variant, L6I, elicits functional responses to a09

SUBMITTER: Murugesan G 

PROVIDER: S-EPMC11584656 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature