ATP dynamics as a predictor of future podocyte structure and function after acute ischemic kidney injury in female mice.

ABSTRACT: Acute kidney injury (AKI), typically caused by ischemia, is a common clinical complication with a poor prognosis. Although proteinuria is an important prognostic indicator of AKI, the underlying causal mechanism remains unclear. In vitro studies suggest that podocytes have high ATP demands to maintain their structure and function, however, analyzing their ATP dynamics in living kidneys has been technically challenging. Here, using intravital imaging to visualize a FRET-based ATP biosensor expressed systemically in female mice due to their suitability for glomerular imaging, we monitor the in vivo ATP dynamics in podocytes during ischemia reperfusion injury. ATP levels decrease during ischemia, but recover after reperfusion in podocytes, exhibiting better recovery than in glomerular endothe

SUBMITTER: Takahashi M 

PROVIDER: S-EPMC11584722 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature