Ectopic expression of choline oxidase (<i>codA</i>) gene from <i>Arthrobacter globiformis</i> confers drought stress tolerance in transgenic sugarcane.

ABSTRACT: Drought is a serious problem that impacts sugarcane production and productivity worldwide. In this current investigation, a codon-optimized choline oxidase (codA) gene was transformed into Saccharum hybrid cultivar Co 86032 through Agrobacterium-mediated transformation. The transgenic events with the codA gene driven by the portubi882 (PD2) promoter accumulated elevated levels of glycine betaine (5 - 10µg/g) whereas untransformed control plants accumulated less than 1.5µg/g which in turn maintained the plant health by sustaining transpiration rate (4 - 5 µmol of H₂O/cm²/s) and photosynthetic efficiency (30 - 34 µmol/Co₂/s) whereas the control plants suffered from 50% reduction under water-deficit stress condition. Morpho-anatomic

SUBMITTER: Chinnaswamy A 

PROVIDER: S-EPMC11584842 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature