Investigating the Tolerance of Corneal Endothelial Cells to Surgical Fluid Pressure Using Intact Porcine Eyes.

ABSTRACT:

Purpose

It remains unclear if fluid pressures used during cataract surgeries contribute to iatrogenic corneal endothelial cell (CEC) loss.

Methods

A custom experimental platform was used to pressurize the anterior chamber of explanted porcine eyes to surgical fluid pressures of 60 mm Hg or 400 mm Hg for 5 minutes or 60 mm Hg for 45 minutes (n = 8 or 9 per group). The corneal endothelia were stained with a unique combination of nucleic acid viability dyes and were imaged using fluorescence microscopy without removing the cornea from the globe. The images were analyzed using custom code to quantify acute CEC loss as a percentage of CEC injury/death (PCI). The PCI values from the surgical pressure groups were compared to sham controls perfused to physiological pressures for eac

SUBMITTER: McMullen AJ 

PROVIDER: S-EPMC11585060 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature