Thymidine phosphorylase mediates SARS-CoV-2 spike protein enhanced thrombosis in K18-hACE2^TG mice.

ABSTRACT:

Introduction

Thymidine phosphorylase (TYMP), which facilitates platelet activation and thrombosis, is significantly increased in COVID-19 patients. We hypothesize that TYMP mediates SARS-CoV-2 spike protein (SP)-induced thrombosis.

Materials and methods

Plasmids encoding wildtype SP or empty vector (p3.1) were transfected into COS-7 cells, and cell lysates were prepared as a reservoir for SP or p3.1 (control), respectively. K18-hACE2^TG and K18-hACE2^TG/Tymp^-/- mice were treated with a single dose of SP or p3.1 by intraperitoneal injection and then subjected to thrombosis studies three days later. The role of SP on inflammatory signaling activation was assessed in BEAS-2B cells.

Results

SARS-CoV-2 SP increased the expression of TYMP, res

SUBMITTER: Roytenberg R 

PROVIDER: S-EPMC11585440 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature