A customizable and low-cost 3D-printed transwell device coupled with 3D cell culture for permeability assay.

ABSTRACT: The permeability-based assay is commonly used to assess intestinal barrier function, and it relies on using a transwell insert as an essential compartment. The device consists of a semipermeable membrane that is attached at the bottom of the insert and splits the system into the apical and basolateral compartments. However, commercial inserts are standardized with different pore sizes based on the application and offer only a flat plane of two-dimensional cell culture. Herein, we present a simple, low-cost 3D-printed transwell device and a robust method to functionalize the inserts for paper-based 3D cell culture. This 3D-printed device was fabricated from a polylactic acid (PLA) filament, and a paper membrane used to support HT-29 cells for intestinal permeability assessment. A device sho

SUBMITTER: Supjaroen P 

PROVIDER: S-EPMC11585668 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature