The two major splice variants of scavenger receptor BI differ by their interactions with lipoproteins and cellular localization in endothelial cells.

ABSTRACT: The scavenger receptor BI (SR-BI) facilitates the transport of both HDL and LDL through endothelial cells. Its two splice variants, SR-BI_var1 and SR-BI_var2, differ in their carboxy terminal domains. Only SR-BI_var1 contains the putative binding sites for the adapter proteins PDZ domain containing protein 1 (PDZK1) and dedicator of cytokinesis 4 (DOCK4), which limit the cell surface abundance and internalization of the receptor. To investigate the cellular localization of the SR-BI variants and their interaction with lipoproteins in endothelial cells, EA.hy926 cells were stably transfected with vectors encoding untagged, GFP- or mCherry-tagged constructs of the two SR-BI variants. Additionally, the cells were transfected with shRNAs against PDZK1 or DOCK4. Mi

SUBMITTER: Potapenko A 

PROVIDER: S-EPMC11585690 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature