Multilevel Proteomics Reveals Epigenetic Signatures in BCG-Mediated Macrophage Activation.

ABSTRACT: The bacillus Calmette-Guérin BCG vaccine (Mycobacterium bovis) is primarily used to prevent tuberculosis (TB) infections but has wide-ranging immunogenic effects. One of its most notable properties is its ability to induce trained immunity, a memory-like response in innate immune cells such as macrophages. Through targeted analyses of well-established histone marks, prior research has shown that these changes are generated through epigenetic modification. Mass spectrometry-based proteomic approaches provide a way to globally profile various aspects of the proteome, providing data to further identify unexplored mechanisms of BCG-mediated immunomodulation. Here we use multi-level proteomics (total, histone, and phospho to identify networks and potential mechanisms that mediate BCG-induced im

SUBMITTER: Schaefer Z 

PROVIDER: S-EPMC11585779 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature