Ocular tear fluid biomarkers collected by contact lenses.

ABSTRACT:

Purpose

To collect tear fluid biomarkers from contact lenses (CLs) and determine the impact of CL wear duration.

Methods

Rabbits were fitted with commercial etafilcon A CLs, which were collected after 1 min, 4 and 8 h (n = 4/time point). Tear fluid proteins and cytokines were extracted from the CLs and quantified. An exploratory comparison was performed between CLs and Schirmer Strips (SS) for a 1 min duration.

Results

The concentration of MUC5AC was significantly higher after 4 h of CL wear. The expression of all investigated cytokines (IL-1α, IL-1β, IL-8, IL-17A, IL-21, Leptin, MIP-1β, MMP-9, NCAM-1, and TNF-α) was detectable after 1 min of CL wear, and over time, all showed significant variations throughout the 8-h CL wear period. Notably, IL-1α significantly incre

SUBMITTER: Boychev N 

PROVIDER: S-EPMC11586051 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature