An engineered Japanese encephalitis virus mRNA-lipid nanoparticle immunization induces protective immunity in mice.

ABSTRACT:

Introduction

Japanese encephalitis virus (JEV) and Zika virus (ZIKV) pose a severe threat to human health. Our previous research results, as well as those of other research groups, indicated that antibodies (Abs) induced by JEV infection or JEV vaccine vaccination could enhance ZIKV infection in vitro and exacerbate the mortality of ZIKV-infected mice, vice versa, which is known as antibody-dependent enhancement (ADE). Although studies on other flaviviruses revealed that altering the amino acid residues located in the fusion loop (FL) of envelope (E) protein can reduce the level of flavivirus-cross-reactive Abs, thereby abating the ADE of heterologous flavivirus infection, it is unclear whether this strategy is equally applicable to JEV.

Methods

In this study, we cons

SUBMITTER: Zhu J 

PROVIDER: S-EPMC11586386 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature