Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite.

ABSTRACT: Approaches to DNA extraction play a crucial role in determining the variability of results obtained through 16S rRNA amplicon sequencing. Particularly, clay-rich samples can impede the efficiency of various standard cultivation-independent techniques. We conducted an inter-laboratory comparison study to thoroughly assess the efficacy of two published DNA extraction methods (kit-based and phenol-chloroform-based) specifically designed for bentonite samples. To this end, we spiked Wyoming MX 80 bentonite with two different mock communities and compared the obtained DNA yield and purity, the presence of contaminants and the community profile. Our findings suggest that both methods are equally viable, with the best choice depending on the specific requirements of the downstream analysis. Howev

SUBMITTER: Mijnendonckx K 

PROVIDER: S-EPMC11586505 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature