Comprehensive gene expression analysis in gallbladder mucosal epithelial cells of dogs with gallbladder mucocele.

ABSTRACT:

Background

Gallbladder mucocele (GBM) is a common disease in the canine gallbladder. Although the pathogenesis of GBM remains unclear, we recently reported that the excessive accumulation of mucin in the gallbladder is not a result of overproduction by gallbladder epithelial cells (GBECs).

Hypothesis/objectives

Changes in the function of GBECs other than the production of mucin are associated with the pathogenesis of GBM. We performed an RNA sequencing (RNA-seq) analysis to comprehensively search for abnormalities in gene expression profiles of GBECs in dogs with GBM.

Animals

Fifteen dogs with GBM and 8 dogs euthanized for reasons other than gallbladder disease were included.

Methods

The GBECs were isolated from gallbladder tissues to extract RNA. The RNA-seq a

SUBMITTER: Nagao I 

PROVIDER: S-EPMC11586547 | biostudies-literature | 2024 Nov-Dec

REPOSITORIES: biostudies-literature