Structure-function mapping and mechanistic insights on the SARS CoV2 Nsp1.

ABSTRACT: Non-structural protein 1 (Nsp1) is a key component of the infectious process caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV2), responsible for the COVID-19 pandemic. Our previous data demonstrated that Nsp1 can degrade both RNA and DNA in the absence of the ribosome, a process dependent on the metal ions Mn²⁺, Ca²⁺, or Mg²⁺ (Salgueiro et al., SARS-CoV2 Nsp1 is a metal-dependent DNA and RNA endonuclease. Biometals. 2024;37:1127-1146). The protein is composed of two structural domains: the N-terminal domain (NTD) and C-terminal domain (CTD), connected by a loop. To elucidate the function of each structural domain, we generated four truncated versions of Nsp1 containing either the NTD or the CTD. Our results indicate that the Nsp1

SUBMITTER: Salgueiro BA 

PROVIDER: S-EPMC11586866 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature