Digital quantitation of bridging fibrosis and septa reveals changes in natural history and treatment not seen with conventional histology.

ABSTRACT:

Background and aims

Metabolic dysfunction-associated steatohepatitis (MASH) with bridging fibrosis is a critical stage in the evolution of fatty liver disease. Second harmonic generation/two-photon excitation fluorescence (SHG/TPEF) microscopy with artificial intelligence (AI) provides sensitive and reproducible quantitation of liver fibrosis. This methodology was applied to gain an in-depth understanding of intra-stage fibrosis changes and septa analyses in a homogenous, well-characterised group with MASH F3 fibrosis.

Methods

Paired liver biopsies (baseline [BL] and end of treatment [EOT]) of 57 patients (placebo, n = 17 and tropifexor n = 40), with F3 fibrosis stage at BL according to the clinical research network (CRN) scoring, were included. Unstained sections were exami

SUBMITTER: Naoumov NV 

PROVIDER: S-EPMC11586893 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature