Engineered uterine primary myometrial cells with high-mobility group AT-hook 2 overexpression display a leiomyoma-like transcriptional and epigenomic phenotype.

ABSTRACT:

Objective

To determine if engineered high-mobility group AT-hook 2 (HMGA2) overexpressing uterine primary myometrial cells recapitulate the transcriptional and epigenomic features of HMGA2-subtype leiomyomas.

Design

Isolated primary, "normal" myometrial cells from three patients were engineered to overexpress HMGA2 to determine how HMGA2 establishes transcriptomic and epigenomic features of HMGA2-overexpressing leiomyoma.

Setting

Academic research laboratory.

Patient(s)

Primary myometrial cells were isolated from normal myometrium obtained from three patients undergoing hysterectomy.

Intervention(s)

Not applicable.

Main outcome measure(s)

Determined genome-wide transcriptomic and epigenomic features of engineered HMGA2-overexpressing uterine prima

SUBMITTER: Saini P 

PROVIDER: S-EPMC11588529 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature