HMGA2 overexpression induces plasticity in myometrial cells and a transcriptomic profile more similar to that of uterine fibroids.

ABSTRACT:

Objective

To study the possible role for HMGA2 overexpression in differentiated myometrial cells and its potential to induce a stem cell-like or dedifferentiating phenotype and drive fibroid development.

Design

Myometrial cells were immortalized and transduced with an HMGA2 lentivirus to produce HMGA2hi cells. In vitro stem cell assays were conducted, and ribonucleic acid from HMGA2hi and control cells as well as fibroid-free myometrial and HMGA2 fibroid (HMGA2F) tissues were submitted for ribonucleic acid sequencing.

Setting

University research laboratory.

Patient(s)

Women who underwent hysterectomy for symptomatic uterine fibroids or other gynecological conditions.

Intervention(s)

Not applicable.

Main outcome measure(s)

In vitro stem cell-like p

SUBMITTER: Paul EN 

PROVIDER: S-EPMC11588543 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature