Genomic characterization of a bla _KPC-2-producing IncM2 plasmid harboring transposon ΔTn6296 in Klebsiella michiganensis.

ABSTRACT: Klebsiella michiganensis is an emerging hospital-acquired bacterial pathogen, particularly strains harboring plasmid-mediated carbapenemase genes. Here, we recovered and characterized a multidrug-resistant strain, bla _KPC-2-producing Klebsiella michiganensis LS81, which was isolated from the abdominal drainage fluid of a clinical patient in China, and further characterized the co-harboring plasmid. K. michiganensis LS81 tested positive for the bla _KPC-2 genes by PCR sequencing, with bla _KPC-2 located on a plasmid as confirmed by S1 nuclease pulsed-field gel electrophoresis combined with Southern blotting. In the transconjugants, the bla _KPC-2 genes were successfully transferred to the recipient str

SUBMITTER: Song JM 

PROVIDER: S-EPMC11588702 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature