Dataset Information

Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σ^D and σ^H deciphered using computer modeling and point mutagenesis.

ABSTRACT: This study aimed to reveal interactions of the stress response sigma subunits (factors) σ^D and σ^H of RNA polymerase and promoters in Gram-positive bacterium Corynebacterium glutamicum by combining wet-lab obtained data and in silico modeling. Computer modeling-guided point mutagenesis of C. glutamicum σ^H subunit led to the creation of a panel of σ^H variants. Their ability to initiate transcription from naturally occurring hybrid σ^D/σ^H-dependent promoter Pcg0441 and two control canonical promoters (σ^D-dependent PrsdA and σ^H-dependent PuvrD3) was measured and interpreted using molecular dynamics simulations of homology models of all complexes. The results led us to design the artificial hybrid promoter PD

SUBMITTER: Blumenstein J

PROVIDER: S-EPMC11588781 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Dataset Information

Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σ^D and σ^H deciphered using computer modeling and point mutagenesis.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σD and σH deciphered using computer modeling and point mutagenesis.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σ^D and σ^H deciphered using computer modeling and point mutagenesis.