Cucumber Green Mottle Mosaic Virus Coat Protein Hijacks Mitochondrial ATPδ to Promote Viral Infection.

ABSTRACT: The production and scavenging of reactive oxygen species (ROS) are critical for plants to adapt to biotic and abiotic stresses. In this study, we investigated the interaction between the coat protein (CP) of cucumber green mottle mosaic virus (CGMMV) and ATP synthase subunit δ (ATPδ) in mitochondria. Silencing of ATPδ by tobacco rattle virus-based virus-induced gene silencing impeded CGMMV accumulation in Nicotiana benthamiana leaves. Both the overexpression of ATPδ in transgenic plants and transient expression promoted CGMMV infection. Nitro blue tetrazolium (NBT) and 3,3'-diaminobenzidine (DAB) staining revealed that ATPδ inhibited O₂ ^- production but not H₂O₂ production. The treatment of CGMMV-infected leaves with the ROS inhibitor diphenylene

SUBMITTER: Yang X 

PROVIDER: S-EPMC11588859 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature