Long-term (10-year) monitoring of transposon-mediated transgenic cattle.

ABSTRACT: The production of transgenic animals using non-viral methods has raised questions regarding their long-term health and genomic stability. In this study, we evaluated these aspects in transgenic cattle over ten years, using transposon-mediated gene transfer. Our longitudinal analysis included a comprehensive health assessment and whole-genome DNA resequencing. We found no significant alterations in physiological parameters or health complications in transposon-mediated transgenic cattle that exceeded 10 years of age. Genomic analysis revealed that the rates of somatic mutations and copy number variations in transgenic cattle were comparable to those in non-transgenic cattle. Furthermore, structural variants were infrequent, suggesting that transposon-mediated gene insertion did not compromi

SUBMITTER: Yum SY 

PROVIDER: S-EPMC11588892 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature