High-throughput genotyping of Plasmodium vivax in the Peruvian Amazon via molecular inversion probes.

ABSTRACT: Plasmodium vivax transmission occurs throughout the tropics and is an emerging threat in areas of Plasmodium falciparum decline, causing relapse infections that complicate treatment and control. Targeted sequencing for P. falciparum has been widely deployed to detect population structure and the geographic spread of antimalarial and diagnostic resistance. However, there are fewer such tools for P. vivax. Leveraging global variation data, we designed four molecular inversion probe (MIP) genotyping panels targeting geographically differentiating SNPs, neutral SNPs, putative antimalarial resistance genes, and vaccine candidate genes. We deployed these MIP panels on 866 infections from the Peruvian Amazon and identified transmission networks with clonality (IBD[identity by descent]>0.99), copy

SUBMITTER: Popkin-Hall ZR 

PROVIDER: S-EPMC11589703 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature