Antibody binding and neutralization of primary and T-cell line-adapted isolates of human immunodeficiency virus type 1.

ABSTRACT: The relative resistance of human immunodeficiency virus type 1 (HIV-1) primary isolates (PIs) to neutralization by a wide range of antibodies remains a theoretical and practical barrier to the development of an effective HIV vaccine. One model to account for the differential neutralization sensitivity between Pls and laboratory (or T-cell line-adapted [TCLA]) strains of HIV suggests that the envelope protein (Env) complex is made more accessible to antibody binding as a consequence of adaptation to growth in established cell lines. Here, we revisit this question using genetically related PI and TCLA viruses and molecularly cloned env genes. By using complementary techniques of flow cytometry and virion binding assays, we show that monoclonal antibodies targeting the V3 loop, CD4-binding si

SUBMITTER: York J 

PROVIDER: S-EPMC115898 | biostudies-literature | 2001 Mar

REPOSITORIES: biostudies-literature