TAT as a new marker and its use for noninvasive chemical biopsy in NASH diagnosis.

ABSTRACT:

Background

Early diagnosis of Nonalcoholic steatohepatitis (NASH) is crucial to prevent its progression to hepatocellular carcinoma, but its gold standard diagnosis still requires invasive biopsy. Here, a new marker-based noninvasive chemical biopsy approach is introduced that uses urine-secreted tyrosine metabolites.

Methods

We first identified NASH-specific decrease in TAT expression, the first enzyme in the tyrosine degradation pathway (TDP), by employing exometabolome-transcriptome correlations, single-cell RNA -seq, and tissue staining on human NASH patient samples. A selective extrahepatic monitoring of the TAT activity was established by the chemical biopsy exploiting the enzyme's metabolic conversion of D₂-tyrosine into D₂-4HPP. The approach was

SUBMITTER: Jo S 

PROVIDER: S-EPMC11590374 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature