GnuR Represses the Expression of Glucose and Gluconate Catabolism in Pseudomonas putida KT2440.

ABSTRACT: In Pseudomonas putida KT2440, a prime chassis for biotechnology, the clustered distribution of glucose catabolism genes and four related transcription factors (TFs) may facilitate the tight regulation of glucose catabolism. However, the genes under the direct control of these TFs remain unidentified, leaving their regulatory roles elusive. Furthermore, the carbon source gluconate was metabolised similarly to glucose in KT2440, but the responses of these catabolic and TF genes to gluconate were unclear. Here, these mysteries were unravelled through multi-omics analysis integrated with physiological studies. First, we found that the expression of these catabolic and TF genes were significantly induced by both glucose and gluconate in KT2440. The independent responses of these genes to glucos

SUBMITTER: Chen W 

PROVIDER: S-EPMC11590683 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature