Protective Effects of 17-βE₂ on the Primary Hepatocytes of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Under Acute Heat Stress.

ABSTRACT: The rainbow trout (Oncorhynchus mykiss) is a typical cold-water species. However, due to global warming, it has experienced prolonged high-temperature stress. Research indicates that thermotolerance in rainbow trout varies by sex at multiple physiological levels. Specifically, females exhibit higher thermotolerance, which may be attributed to estrogen-mediated signal transduction pathways. This study involved culturing primary hepatocytes from rainbow trout and exposing them to estradiol and estrogen receptor antagonists to assess estradiol's protective effects. The analysis focused on expression of ER, HSPs genes, hepatocyte viability, and antioxidant indices. Four experimental groups were treated with 17-βE₂ at concentrations of 0, 0.1, 1, and 10 μM/mL for durations of

SUBMITTER: Zhao G 

PROVIDER: S-EPMC11590922 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature