Development of Microsatellite Markers from Transcriptome of Eriocheir sinensis and Their Application in Multiplex PCR Panels.

ABSTRACT:

Background

The development of genetic markers is crucial for aquaculture and population genetics. Polyallelic microsatellite markers are widely used for breeding and family management of Eriocheir sinensis (E. sinensis), contributing to the sustainable development of the industry.

Methods

Through transcriptome analysis, 100 highly polymorphic SSR markers were identified and developed. From these, four multiplex PCR panels, each containing 16 markers, were constructed and applied to parentage assignment in six E. sinensis families.

Results

The number of alleles (N_a) ranged from 7 to 21, the observed heterozygosity (H_o) varied from 0.356 to 0.951, and the expected heterozygosity (H_e) varied from

SUBMITTER: Xiao Q 

PROVIDER: S-EPMC11590995 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature