Exploring the Interactome of the Queuine Salvage Protein DUF2419 in <i>Entamoeba histolytica</i>.

ABSTRACT: Entamoeba histolytica causes amebiasis, a significant global health issue, with millions affected annually, especially in developing countries. EhDUF2419, an important protein involved in E. histolytica's queuine salvage pathway and its interaction network, remains unclear. To explore this, we transfected E. histolytica trophozoites with a plasmid encoding Myc-tagged EhDUF2419 and achieved successful overexpression. Through immunoprecipitation with the Myc antibody followed by mass spectrometry, we identified 335 proteins interacting with Myc-tagged EhDUF2419, including over 100 ribosomal proteins, along with translation initiation and elongation factors, and aminoacyl-tRNA synthetases. Ribosome purification revealed the presence of EhDUF2419 in ribosomal protein-enric

SUBMITTER: Ye J 

PROVIDER: S-EPMC11592518 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature