A New Approach of Detecting ALK Fusion Oncogenes by RNA Sequencing Exon Coverage Analysis.

ABSTRACT:

Background

In clinical practice, various methods are used to identify ALK gene rearrangements in tumor samples, ranging from "classic" techniques, such as IHC, FISH, and RT-qPCR, to more advanced highly multiplexed approaches, such as NanoString technology and NGS panels. Each of these methods has its own advantages and disadvantages, but they share the drawback of detecting only a restricted (although sometimes quite extensive) set of preselected biomarkers. At the same time, whole transcriptome sequencing (WTS, RNAseq) can, in principle, be used to detect gene fusions while simultaneously analyzing an incomparably wide range of tumor characteristics. However, WTS is not widely used in practice due to purely analytical limitations and the high complexity of bioinformatic an

SUBMITTER: Zakharova G 

PROVIDER: S-EPMC11592821 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature