A Novel Method to Profile Transcripts Encoding SH2 Domains in the <i>Patiria miniata</i> Mature Egg Transcriptome.

ABSTRACT: The critical mechanism to restart zygote metabolism and prevent polyspermy during fertilization is the intracellular Ca²⁺ increase. All of the signaling molecules leading to the Ca²⁺ rise are not fully known in any species. In the sea star Patiria miniata, SFK1, SFK3, and PLCγ participate in this fertilization Ca²⁺ increase. These proteins share common regulatory features, including signaling via tyrosine phosphorylation and their SH2 domains. In this study, we explore two different bioinformatic strategies to identify transcripts in the Patiria miniata mature egg transcriptome (Accession PRJNA398668) that code for proteins possessing an SH2 domain. The first identified the longest open reading frame for each transcript and then utilized simil

SUBMITTER: Bates L 

PROVIDER: S-EPMC11593052 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature