Enzymatic Oxidation of Aflatoxin M₁ in Milk Using CotA Laccase.

ABSTRACT: Aflatoxin M₁ (AFM₁) in milk poses a significant threat to human health. This study examined the capacity of Bacillus licheniformis CotA laccase to oxidize AFM₁. The optimal conditions for the CotA laccase-catalyzed AFM₁ oxidation were observed at pH 8.0 and 70 °C, achieving an AFM₁ oxidation rate of 86% in 30 min. The Km and Vmax values for CotA laccase with respect to AFM₁ were 18.91 μg mL^-1 and 9.968 μg min^-1 mg^-1, respectively. Computational analysis suggested that AFM₁ interacted with CotA laccase via hydrogen bonding and van der Waals interactions. Moreover, the oxidation products of AFM₁ mediated by CotA laccase were identified as the C3-hydroxy deriv

SUBMITTER: Guo Y 

PROVIDER: S-EPMC11593616 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature