Biophysical Analysis of Vip3Aa Toxin Mutants Before and After Activation.

ABSTRACT: Cry toxins from Bacillus thuringiensis are effective biopesticides that kill lepidopteran pests, replacing chemical pesticides that indiscriminately attack both target and non-target organisms. However, resistance in susceptible pests is an emerging problem. B. thuringiensis also produces vegetative insecticidal protein (Vip3A), which can kill insect targets in the same group as Cry toxins but using different host receptors, making the combined application of Cry and Vip3A an exciting possibility. Vip3A toxicity requires the formation of a homotetramer. Hence, screening of Vip3A mutants for increased stability requires orthogonal biophysical assays that can test both tetrameric integrity and monomeric robustness. For this purpose, we have used herein for the first time a comb

SUBMITTER: Khunrach P 

PROVIDER: S-EPMC11594144 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature