A Novel Single Base Mutation in OsSPL42 Leads to the Formation of Leaf Lesions in Rice.

ABSTRACT: Rice spotted-leaf mutants serve as valuable resources for studying plant programmed cell death (PCD) and disease resistance mechanisms, making them crucial for research on disease resistance in rice. Map-based cloning was used to identify and clone the spotted-leaf gene OsSPL42. Then, functional complementation and CRISPR/Cas9 techniques were also employed to further validate the function of this gene. By applying leaf clippings for bacterial blight (BB) inoculation, the BB resistance of different rice lines was assessed. The results in this study were as follows: The OsSPL42 behaved as a recessive nuclear gene and was narrowed down to a 111 kb region on chromosome 8. All T₀ transgenic rice plants in the complementation experiments exhibited a wild-type phenotype,

SUBMITTER: Li P 

PROVIDER: S-EPMC11594205 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature