The pesticides carbofuran and picloram alter the diversity and abundance of soil microbial communities.

ABSTRACT: Many countries widely use pesticides to increase crop productivity in agriculture. However, their excessive and indiscriminate use contaminates soil and other environments and affects edaphic microbial communities. We aimed to examine how the pesticides carbofuran and picloram affect the structure and functionality of soil microbiota using cultivation-independent methods. Total DNA was extracted from microcosms (treated or not with pesticides) for amplification and metabarcoding sequencing for bacteria (16S gene) and fungi (28S gene) using Illumina-MiSeq platform. Data analysis resulted in 6,772,547 valid reads from the sequencing, including 3,450,815 amplicon sequences from the V3-V4 regions of the 16S gene and 3,321,732 sequences from the 28S gene. A total of 118 archaea, 6,931 bacteria,

SUBMITTER: Alves Senabio J 

PROVIDER: S-EPMC11594414 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature