Differential Dynamics and Roles of FKBP51 Isoforms and Their Implications for Targeted Therapies.

ABSTRACT: The expression of FKBP5, and its resulting protein FKBP51, is strongly induced by glucocorticoids. Numerous studies have explored their involvement in a plethora of cellular processes and diseases. There is, however, a lack of knowledge on the role of the different RNA splicing variants and the two protein isoforms, one missing functional C-terminal motifs. In this study, we use in vitro models (HeLa and Jurkat cells) as well as peripheral blood cells of a human cohort (N = 26 male healthy controls) to show that the two expressed variants are both dynamically upregulated following dexamethasone, with significantly earlier increases (starting 1-2 h after stimulation) in the short isoform both in vitro and in vivo. Protein degradation assays in vitro showed a reduced half-life

SUBMITTER: Martinelli S 

PROVIDER: S-EPMC11594789 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature