Functional Characterization of the 14-3-3 Gene Family in Alfalfa and the Role of MsGRF2 in Drought Response Mechanisms.

ABSTRACT: Drought stress affects crop growth and development, significantly reducing crop yield and quality. Alfalfa (Medicago sativa L.), the most widely cultivated forage crop, is particularly susceptible to drought. The general regulatory factor (GRF) protein 14-3-3, a highly conserved family in plants, specifically recognizes and binds to phosphoserine residues in target proteins, regulating both plant development and responses to environmental stressors. In this study, 66 alfalfa 14-3-3 proteins were identified, and the full-length MsGRF2 gene was cloned and functionally analyzed. The expression of MsGRF2 was highest in alfalfa inflorescences and lowest in roots. Transgenic tobacco overexpressing MsGRF2 exhibited increased tolerance to low temperature and drought str

SUBMITTER: Chai L 

PROVIDER: S-EPMC11595020 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature