Identification of Host Factors Interacting with Movement Proteins of the 30K Family in Nicotiana tabacum.

ABSTRACT: The interaction of viral proteins with host factors represents a crucial aspect of the infection process in plants. In this work, we developed a strategy to identify host factors in Nicotiana tabacum that interact with movement proteins (MPs) of the 30K family, a group of viral proteins around 30 kDa related to the MP of tobacco mosaic virus, which enables virus movement between plant cells. Using the alfalfa mosaic virus (AMV) MP as a model, we incorporated tags into its coding sequence, without affecting its functionality, enabling the identification of 121 potential interactors through in vivo immunoprecipitation of the tagged MP. Further analysis of five selected candidates (histone 2B (H2B), actin, 14-3-3A protein, eukaryotic initiation factor 4A (elF4A), and a peroxidase-POX-)

SUBMITTER: Villar-Alvarez D 

PROVIDER: S-EPMC11595209 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature