Strategy for Optimizing Vitamin B12 Production in Pseudomonas putida KT2440 Using Metabolic Modeling.

ABSTRACT: Background/Objectives: Vitamin B₁₂ is very important for human health, as it is a cofactor for enzymatic activities and plays various roles in human physiology. It is highly valued in the pharmaceutical, food, and additive production industries. Some of the bacteria currently used for the vitamin production are difficult to modify with gene-editing tools and may have slow growth. We propose the use of the bacteria Pseudomonas putida KT2440 for the production of vitamin B₁₂ because it has a robust chassis for genetic modifications. The present wok evaluates P. putida KT2440 as a host for vitamin B₁₂ production and explore potential gene-editing optimization strategies. Methods: We curated and modified a genome-scale metabolic mode

SUBMITTER: Prieto-de Lima TS 

PROVIDER: S-EPMC11596459 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature