Isolation, Characterization, and Genome Engineering of a Lytic Pseudomonas aeruginosa Phage.

ABSTRACT: Antibiotic-resistant bacterial infections have become one of the leading causes of human mortality. Bacteriophages presented great potential for combating antibiotic-resistant infections in the post-antibiotic era due to their high host specificity and safety profile. Pseudomonas aeruginosa, an opportunistic pathogenic bacterium, has shown a surge in multidrug-resistant strains, severely impacting both human health and livestock. In this study, we successfully isolated and purified a P. aeruginosa-specific phage, PpY1, from feces collected from a breeding farm. This phage harbors a short tail and a 43,787 bp linear genome, and exhibited potent lytic activity against several pathogenic P. aeruginosa strains. Leveraging Transformation-associated recombination (TAR

SUBMITTER: Cong X 

PROVIDER: S-EPMC11596505 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature