Zic-HILIC MS/MS Method for NADomics Provides Novel Insights into Redox Homeostasis in Escherichia coli BL21 Under Microaerobic and Anaerobic Conditions.

ABSTRACT: Background: Nicotinamide adenine dinucleotide (NAD⁺), its precursors, and its derivatives (collectively NADome) play a crucial role in cellular processes and maintain redox homeostasis. Understanding the dynamics of these metabolic pools and redox reactions can provide valuable insights into metabolic functions, especially cellular regulation and stress response mechanisms. The accurate quantification of these metabolites is challenging due to the interconversion between the redox forms. Methods: Our laboratory previously developed a zwitterionic hydrophilic interaction liquid chromatography (zic-HILIC)-tandem mass spectrometry method for the quantification of five essential pyridine nucleotides, including NAD⁺ derivatives and it's reduced forms, with

SUBMITTER: Rane DV 

PROVIDER: S-EPMC11596675 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature