Oxford Nanopore Technology-Based Identification of an <i>Acanthamoeba castellanii</i> Endosymbiosis in Microbial Keratitis.

ABSTRACT: (1) Background: Microbial keratitis is a serious eye infection that carries a significant risk of vision loss. Acanthamoeba spp. are known to cause keratitis and their bacterial endosymbionts can increase virulence and/or treatment resistance and thus significantly worsen the course of the disease. (2) Methods and Results: In a suspected case of Acanthamoeba keratitis, in addition to Acanthamoeba spp., an endosymbiont of acanthamoebae belonging to the taxonomic order of Holosporales was detected by chance in a bacterial 16S rDNA-based pan-PCR and subsequently classified as Candidatus Paracaedibacter symbiosus through an analysis of an enlarged 16S rDNA region. We used Oxford Nanopore Technology to evaluate the usefulness of whole-genome sequencing (WGS) as a one-step d

SUBMITTER: Scharf SA 

PROVIDER: S-EPMC11596929 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature