Efficacy of UV-C 254 nm Light and a Sporicidal Surface Disinfectant in Inactivating Spores from <i>Clostridioides difficile</i> Ribotypes In Vitro.

ABSTRACT: Clostridioides difficile is widely recognised as one of the most common causes of healthcare-associated C. difficile infections due to the ability of spores to survive for prolonged periods in the hospital environment. This study aimed to evaluate the efficacy of UV-C 254 nm light in the inactivation of the spores of different C. difficile ribotypes on brain heart infusion (BHI) agar plates or in phosphate-buffered saline (PBS) with varying spore densities. Furthermore, the effectiveness of a sporicidal surface disinfectant against C. difficile spores was determined on different surfaces. Spore suspensions of different C. difficile strains in the range of 10⁵-10⁷ colony-forming units (CFUs) mL^-1 were inoculated on BHI agar

SUBMITTER: Blau K 

PROVIDER: S-EPMC11597166 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature