Method to Generate Chlorine Dioxide Gas In Situ for Sterilization of Automated Incubators.

ABSTRACT: Pharmaceutical preclinical tests using cell cultures are nowadays commonly automated. Incubator microbial contaminations impact such tests. Chlorine dioxide (ClO₂) is widely used in aqueous solutions. However, a gaseous form, such as chlorine dioxide gas (gClO₂), can effectively access unreachable spaces, such as closed cell culture incubators. Steam sterilization requires a temperature rise to at least 121 °C, thus limiting the possibility of automation elements for sensors and actuators. gClO₂ sterilization is an ambient-temperature sterilization method. This article aims to demonstrate that gClO₂ generated from solid powder tablets is efficient for sterilizing incubators and can be automated. We selected (i) Bacillus subtilis strain, (i

SUBMITTER: Schicklin C 

PROVIDER: S-EPMC11597574 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature